Composite

Part:BBa_K3715009:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-27)


T7 promoter+RBS+Linker a+His+Super2+Linker b+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 878
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 863
    Illegal NgoMIV site found at 138
    Illegal NgoMIV site found at 192
    Illegal NgoMIV site found at 219
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized in E. coli BL21.


Source

NCBI

References